TOUR OF FACILITIES AND RESOURCES
   
State-of-the-art instrumentation currently housed at CRBA

ScanArray Express System (shared with CIRB)

DHPLC Wave

MassARRAY Compact Analyzer

CEQ 8000 Xl Genetic Analysis System

Real Time PCR Biorad ICycler

Wallac Victor2 1420 multilabel plate reader

Fluor-S imaging systems for gels and plates

Gas Chromatograph HP 6890 w HP 5973 mass selective detector

HPLC 1090 with UV and Varian ProStarFluorescent detectors

HPLC with 5600A CoulArray electrochemical array detector

Gas chromatograph 3400

Liquid Scintillator Analyzer Packard Tri-Carb 2100 TR

Automatic gamma counter 1272 Clinigamma

Ultracentrifuge Optima

Image cytometer / photomicroscope DMLB Cytometrica

Spot cutter Proteome Works Plus (shared with CIRB)

MALDI-TOF Voyager-DE PRO

ESI-QTOF LC-MS/MS (shared with CIRB)

4000QTRAP LC/MS/MS System

Laser microdissector ÁSL Cut

FACSAria Cell Sorting System

 

Technical Resources empowered at CRBA


* Molecular Biology:

PCR amplification
Real time PCR
Mutation and SNP detection using DHPLC, polarized fluorescence, MALDI-TOF
High throughput SNP analysis
Automated DNA Sequencing
Gel electrophoresis & blotting
Gel Imaging
Hybridization
Microarray analysis

* Proteomics:

Western Blot
2D Gel (shared with CIRB)
Gel Cutter & Digest
Maldi-TOF
ESI-Qtof (shared with CIRB)
Protein and metabolites identification by LC/MS/MS

* In vitro Biophase:

Primary cell cultures
Cellular gene transfection
Bacterial plasmid cloning

* In vivo Biophase:

Autoventilated racks for rodent cages
Surgery and microsurgey on rodents
Isolated Organ perfusion

* Fractionation

Cell fractionation
Sub cellular fractionation
Flow citometry – cell sorting

* Hystology and cytology:

Cyto-Histo preparations
Microtomy
Cryo-microtomy
Enzymatic and molecular cytochemistry
Immunocytochemistry
In situ hybridization
Light microscopy
Laser microdissection
Image cytometry
Microspectrophotometry

* Biochemistry:

Immunochemistry using multilabeled ELISA
Time resolved & fluorescence polarization analyses
Photometry
Gas cromatography - mass spectometry
Radiochemistry
Gas-cromatography
HPLC combined with UV, Fluo & Coulometric detectors

* Bio-informatics and Statistics

 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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